Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms

Figure 5

Dependence of the C_p transition curves of α_D+N-HaH isoforms in buffer 20 mM HEPES (pH 7.06 at 80°C), with a scan rate: 0.2°C min⁻¹ (a); 0.5°C min⁻¹ (b); 1.0°C min⁻¹ (c); 1.5°C min⁻¹ (d). Continuous lines depict the experimental data; dash dot lines show the experimental individual components, result of the annealing process, and symbols represent the result of the sum of the corresponded individual components. In all cases, the protein concentration was 3 mg·ml⁻¹.

(a)

(b)

(c)

(d)