Development of Micellar HPLC-UV Method for Determination of Pharmaceuticals in Water Samples
Table 5
Recovery tests for water samples.
Proposed method
Conventional methods
Sample
Analyte
Recovery (%)
Precision (RSD, %)
Recovery (%)
Precision (RSD, %)
Intraday
Interday
Intraday
Interday
Moscados stream
Caf
99.52
0.94
1.53
98.39
1.21
1.67
Tetra
98.64
1.33
2.89
96.67
2.04
2.55
Cipro
98.92
2.45
3.90
96.20
2.83
4.81
Norflo
99.14
1.09
1.47
98.80
1.55
2.39
Diclo
99.45
1.77
0.95
99.94
0.90
1.96
Ibup
98.20
1.61
2.11
98.85
1.32
1.42
Nime
98.35
2.83
4.54
98.97
2.29
3.11
Ethynyl
97.21
0.97
3.02
99.91
1.48
2.27
Norg
99.08
3.14
4.67
99.87
2.11
2.89
Amox
—
—
—
97.35
1.76
1.53
Cleópatra stream
Caf
97.85
2.37
3.10
98.78
1.05
2.14
Tetra
99.71
0.59
2.48
99.89
1.49
2.95
Cipro
98.22
0.83
1.26
98.96
1.68
3.28
Norflo
101.30
1.11
1.45
100.13
2.27
1.99
Diclo
99.45
0.76
1.90
98.32
1.84
2.56
Ibup
99.60
1.28
2.34
98.88
1.16
1.59
Nime
98.50
0.67
1.19
97.83
2.08
2.31
Ethynyl
99.31
1.04
1.72
98.36
1.53
2.47
Norg
98.06
0.95
1.58
98.97
1.87
2.08
Amox
—
—
—
98.60
1.29
1.84
Borba Gato stream
Caf
98.23
0.61
1.04
98.52
2.08
3.77
Tetra
99.11
1.58
3.27
99.01
1.73
2.01
Cipro
97.89
1.13
1.85
99.27
0.99
1.63
Norflo
98.04
1.20
2.01
99.17
1.62
1.94
Diclo
99.55
0.44
1.33
98.05
1.31
2.22
Ibup
99.19
0.82
1.59
98.82
1.47
1.98
Nime
100.73
0.57
1.98
99.51
0.84
1.72
Ethynyl
99.82
1.28
2.10
98.46
1.10
2.36
Norg
98.44
0.49
1.67
99.04
1.26
1.93
Amox
—
—
—
98.63
0.63
1.69
Mandacarú stream
Caf
100.91
1.23
1.81
98.39
0.55
1.14
Tetra
97.36
1.05
2.17
98.67
1.62
2.51
Cipro
99.94
1.41
2.32
97.20
1.28
1.93
Norflo
99.12
1.95
3.06
98.80
1.99
2.48
Diclo
101.33
1.03
1.90
99.94
2.11
2.83
Ibup
99.60
0.55
1.26
98.85
1.33
1.70
Nime
99.82
0.81
1.78
97.77
0.81
1.66
Ethynyl
99.01
0.90
1.63
99.91
1.55
3.90
Norg
100.29
0.78
1.99
99.87
0.90
1.93
Amox
—
—
—
99.35
1.22
2.07
Morangueiro stream
Caf
99.14
0.32
0.98
98.78
0.95
1.57
Tetra
98.52
0.70
1.53
99.89
0.49
1.00
Cipro
98.05
1.51
2.49
98.96
1.35
2.41
Norflo
98.81
1.63
2.72
100.13
1.21
2.27
Diclo
99.20
0.76
1.44
98.32
0.88
1.82
Ibup
100.33
0.52
0.97
98.88
2.04
2.89
Nime
98.87
1.11
2.06
97.83
1.59
2.70
Ethynyl
99.00
0.83
1.88
98.33
1.11
1.40
Norg
100.94
1.47
2.91
98.97
0.97
1.34
Amox
—
—
—
99.04
0.83
0.90
Maringá stream
Caf
97.99
1.14
2.03
98.52
0.66
1.32
Tetra
99.10
0.95
1.82
99.01
1.01
1.83
Cipro
97.66
1.98
3.06
98.27
1.44
1.68
Norflo
98.34
1.32
2.21
99.17
1.27
2.83
Diclo
99.62
1.77
3.48
99.05
0.59
1.16
Ibup
98.41
0.92
1.85
98.82
1.33
2.29
Nime
99.97
1.06
1.95
98.51
0.78
2.04
Ethynyl
98.55
1.20
1.79
98.46
1.11
1.98
Norg
99.67
1.00
1.53
99.04
1.50
2.10
Amox
—
—
—
98.63
0.95
1.93
Tap water
Caf
99.96
0.25
0.81
100.40
2.05
1.19
Tetra
99.05
2.05
2.97
98.00
1.39
1.72
Cipro
99.31
0.68
1.14
97.93
1.03
1.58
Norflo
99.20
0.92
1.67
98.33
0.76
1.47
Diclo
100.08
0.74
1.93
99.19
0.62
0.95
Ibup
99.19
0.33
0.85
100.71
1.28
1.33
Nime
99.21
0.49
1.36
99.67
0.67
1.59
Ethynyl
99.89
0.97
2.02
97.59
1.04
1.45
Norg
98.40
0.81
1.77
97.71
0.95
1.90
Amox
99.12
1.02
1.84
98.26
1.32
2.34
Data were obtained for water samples spiked with 5.0 µg·L−1 of ibuprofen, 17α-ethynylestradiol, diclofenac, nimesulide, levonorgestrel, ciprofloxacin, and norfloxacin; 10 µg·L−1 of caffeine; and 60 µg·L−1 of amoxicillin. The conventional methods were the same as used in Table 4.
