Speciation Analysis of Arsenic Compounds by HPLC-ICP-MS: Application for Human Serum and Urine

Table 1

Operating conditions of anion-exchange chromatography (AEC) combined with inductively coupled plasma mass spectrometry (ICP-MS) for speciation analysis of arsenic compounds.

Anion-exchange chromatography operating conditions
Pump	Quaternary pump LC 10 A (Shimadzu, Japan) including online degasser, column oven, and system control unit
Column	Hamilton PRP-X100 anion-exchange column (250 × 2.1 mm × 10 µm particle size) with an associated guard column
Column temperature	30°C
Mobile phase	Chanel A: 5 mM (NH4)2CO3, pH 9.0, 0.05% Na2 EDTA
	Chanel B: 50 mM (NH4)2CO3, pH 9.0, 0.05% Na2 EDTA
	Chanel C: MeOH
	Elution mode gradient
Flow rate of mobile phase	400 µL·min^−1
Injection volume	100 µL
ICP-MS operating conditions
ICP-MS system	ELAN 9000 (PerkinElmer Sciex, Penlivia, Canada)
RF powder	1300 W
Plasma gas flow	16 L·min^−1
Auxiliary gas flow	1.25 L·min^−1
Nebulizer gas flow	0.9 L·min^−1
Nebulizer	Cross-flow
Spray chamber	PFA double pass
Monitored ion	As^+ (m/z 74.92)
Dwell time	100 ms
Measurement mode	Peak hopping