SRM-MS Method Development for Hepcidin-25 Peptide

Table 2

A transition list for SRM assay of each of the hepcidin-I and -M containing precursor ion, product ion, and CE values.

Peptide sequence	Precursor ion	Product ion	Dwell time	Collision energy	Fragment
DTHFPICIFCCGCCHRSKCGMCCKT	558.6	763.3	20	16	y21^3+
DTHFPICIFCCGCCHRSKCGMCCKT	558.6	693.3	20	14	y19^3+
DTHFPICIFCCGCCHRSKCGMCCKT	651.6	757.1	20	14	y17^3+
DTHFPICIFCCGCCHRSKCGMCCKT	651.6	708.4	20	14	y16^3+