Analytical Methods Used for the Detection and Quantification of Benzodiazepines

Table 2

Usual limits of detection (LOD), quantification (LOQ), and cutoff of some benzodiazepines by HPLC.

Benzodiazepines	HPLC		Sample type	Reference
	Cutoff (ng/mL)
7-Aminoclonazepam	1		Oral fluid	[11]
Clonazepam	2.5
	LOD (ng/mL)	LOQ (ng/mL)
Nimetazepam	0.25	5	Urine	[17]
7-Aminonimetazepam	1	5
Nitrazepam	0.125	1
Flunitrazepam	0.125	0.25
7-Aminoflunitrazepam	1	2.5
Bromazepam, alprazolam, lorazepam, diazepam	0.01 (ng/μL)	0.03 (ng/μL)	Blood	[16]