A Visible Colorimetric Fluorescent Probe for Hydrogen Sulfide Detection in Wine

Figure 3

(a) Fluorescence spectra of probe 1 (10 μM) with H₂S (0–200 μM); (b) the plot of fluorescence intensity difference with H₂S from 0 to 200 μM in buffer solution (10 mM, pH 4.0) with DMSO (v/v, 3 : 1); (c) fluorescence intensity change of probe 1 (10 μM) upon addition of various species (200 μM for each. 0, blank; 1, GSH; 2, F⁻; 3, Cl⁻; 4, Br⁻; 5, SO₃²⁻; 6, HSO₃⁻; 7, S₂O₃²⁻; 8, S₂O₅²⁻; 9, S₂O₆⁻; 10, CH₃COO⁻; 11, SO₄²⁻; 12, HCO₃⁻; 13, CO₃²⁻. 200 μM for H₂S). Wavelength, 504 nm. The test was repeated 3 times; (d) the solution color of probe 1 with Na₂S and competing species (200 μM for each. 0, H₂S; 1, GSH; 2, F⁻; 3, Cl⁻; 4, Br⁻; 5, SO₃²⁻; 6, HSO₃⁻; 7, S₂O₃²⁻; 8, S₂O₅²⁻; 9, S₂O₆⁻; 10, CH₃COO⁻; 11, SO₄²⁻; 12, HCO₃⁻; 13, CO₃²⁻. 200 μM for H₂S); (e) photograph of probe 1 (10 μM) at different H₂S concentrations under ambient light in buffer solution (pH 4.0) with DMSO (v/v, 3 : 1) at 25°C.

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