Cytotoxic Phenolic Compounds from Fruit Glandular Trichomes of <i>Macaranga tanarius</i>

Doan, Thi Mai Huong; Nguyen, Thuy Linh; Trinh, Thi Thanh Van; Vu, Van Nam; Phi, Thi Dao; Litaudon, Marc; Roussi, Fanny; Chau, Van Minh; Pham, Van Cuong

doi:https://doi.org/10.1155/2019/2917032

Journal of Analytical Methods in Chemistry

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Abstract Introduction Materials and Methods Results and Discussion Conclusion Data Availability Conflicts of Interest Acknowledgments Supplementary Materials References Copyright Related Articles

Research Article | Open Access

Volume 2019 | Article ID 2917032 | https://doi.org/10.1155/2019/2917032

Cytotoxic Phenolic Compounds from Fruit Glandular Trichomes of Macaranga tanarius

Thi Mai Huong Doan,^1,2Thuy Linh Nguyen,¹Thi Thanh Van Trinh,¹Van Nam Vu,¹Thi Dao Phi,¹Marc Litaudon,³Fanny Roussi,³Van Minh Chau,¹and Van Cuong Pham^1,2

Academic Editor: Serban C. Moldoveanu

Received04 Jul 2019

Revised07 Sept 2019

Accepted12 Sept 2019

Published13 Oct 2019

Abstract

A new flavonoid, macatanarin D (1), together with five known stilbenes (2-6), was isolated from fruit glandular trichomes of Macaranga tanarius. Their structures were elucidated on the basis of spectroscopic methods and through comparison with data reported in the literature. All isolated compounds were evaluated for their cytotoxic activities against KB and MCF-7 cell lines. Compounds 3, 4, and 5 showed the strongest activities against both cell lines with IC₅₀ values in the range of 0.03–0.12 μM, and compound 2 only showed a significant cytotoxicity against KB cell line (IC₅₀ = 0.26 μM) and a moderate cytotoxicity against MCF-7 (IC₅₀ = 10.4 μM). Compounds 1 and 6 showed weak cytotoxic activities against KB cell line with IC₅₀ values of 29.3 and 24.7 μM, respectively.

1. Introduction

Macaranga (Euphorbiaceae) is a large genus of about 300 species mainly distributed in Southern Asia, of which 13 species are native to Vietnam [1, 2]. In Vietnam, several species of this genus known as “Ba soi” have been used in traditional medicine for the treatment of swellings, wounds, and diarrhoea [2, 3]. Phytochemical studies of Macaranga species have led to the discovery of various compounds such as flavonoids [4–6] and stilbenes [7, 8], which are regarded as the main constituents [9]. They are responsible for the cytotoxic and antioxidant activities generally found in plants of this genus [9]. Macaranga tanarius is known as “Bach dan nam” in Vietnam. The dried roots are used as an emetic agent, whereas fresh leaves are used as an anti-inflammatory drug to heal wounds [10].

A previous chemical investigation of Macaranga tanarius fruits led to the isolation of seven new and six known prenylated stilbenes [11]. In another study, it was also demonstrated that vedelianin, one of the most potent cytotoxic metabolites of this chemical series, was located in the glandular trichomes present on the surface of fruits of this species [12]. Plant glandular trichomes are considered to be natural cell factories of high biotechnological interest [13]. This result, combined with the highly cytotoxic activity of an AcOEt extract of glandular trichomes of Macaranga tanarius fruits, led us to further investigate chemically these epidermal outgrowths. Herein, we report the isolation of five known prenylated stilbenes (2–6) and the structure elucidation of the new flavonoid macatanarin D (1) and their cytotoxic activities against KB and MCF-7 cancer cell lines.

2. Materials and Methods

2.1. General Experimental Procedures

Optical rotations were determined on a JASCO P-2000 polarimeter (Hachioji, Tokyo, Japan). High-resolution ESIMS was measured on a Varian 910 spectrometer (Varian, California, USA). IR spectra were obtained on a Bruker 23 TENSOR 37 FT-IR spectrometer (Bruker, Billerica, MA, USA). UV spectra were measured using a UV-1601 spectrometer. The ¹H and ¹³C, HMQC, HMBC, NOESY/ROESY, and COSY NMR spectra were recorded on a Bruker AM500 FTNMR spectrometer (Bruker, Billerica, MA, USA), and tetramethylsilane (TMS) was used as an internal standard. Column chromatography (CC) was performed using a silica gel (Kieselgel 60, 70–230 mesh and 230–400 mesh, Merck, Darmstadt, Germany) or Sephadex™ LH-20 (Supelco, Bellefonte, PA, USA). Thin-layer chromatography (TLC) used precoated silica gel 60 F254 (1.05554.0001, Merck, Darmstadt, Germany), and compounds were visualized by spraying with aqueous 10% H₂SO₄ and heating for 1.5–2 min.

2.2. Plant Samples

The fruits of Macaranga tanarius were collected in A Luoi, Thua Thien Hue, Vietnam, in June 2017 and were identified by Dr. Nguyen The Cuong of the Vietnam National Museum of Nature, Vietnam Academy of Science and Technology (VAST). A voucher specimen (VN-2406) was deposited at the Herbarium of the Institute of Ecology and Biological Resources of the Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam. The harvested fruits were carefully dried in a confined space at 40°C for 48 hours. The glandular trichomes were then separated and collected for further investigations by gently hand-rubbing dried fruits on a sieve of stainless-steel mesh.

2.3. Extraction and Isolation

Dry glandular trichomes (200 g) were successively extracted with EtOH (5 × 0.5 L). The extracts were combined and concentrated under diminished pressure. The residue (24 g) was suspended in water (70 mL) and extracted successively with n-hexane and EtOAc. The n-hexane and EtOAc solutions were concentrated under reduced pressure to afford 4.9 g and 10.5 g, respectively. The water solution was concentrated under vacuum to give 7.1 g of dry extract.

The EtOAc extract (10.5 g) was subjected to silica gel column chromatography (CC) eluted with a solvent gradient of CH₂Cl₂/MeOH to yield 9 fractions (F1-F9). F4 (1.5 g) was subjected to a CC on silica gel, eluting with CH₂Cl₂/MeOH gradient to obtain 5 subfractions (F4.1-F4.5). Subfraction F4.3 (137 mg) was purified on a Sephadex LH-20 column (100% MeOH) to yield compound 4 (pale yellow solid, 10.1 mg, yield = 0.005%). Subfraction F4.4 (99.6 mg) was purified on a Sephadex LH-20 column (100% MeOH) and then repurified by CC on silica gel (CH₂Cl₂/MeOH gradient) to provide compound 3 (pale yellow solid, 17.3 mg, yield = 0.0087%) and compound 5 (pale yellow solid, 15.5 mg, yield = 0.0078%). Fraction F6 (2.3 g) was subjected to a CC on silica gel, eluting with CH₂Cl₂/MeOH gradient to obtain 7 subfractions (F6.1-F6.7). Subfraction F6.5 (57 mg) was purified on a Sephadex LH-20 column (100% MeOH) to provide compound 2 (pale yellow solid, 9.2 mg, yield = 0.0046%) and compound 1 (yellow powder, 4.4 mg, yield = 0.0022%). Fraction F7 (1.9 g) was subjected to a CC on silica gel, eluting with CH₂Cl₂/MeOH gradient to obtain 6 subfractions (F7.1-F7.6). Subfraction F7.4 (97 mg) was purified on a Sephadex LH-20 column (CH₂Cl₂/MeOH: 1.5/8.5) to yield compound 6 (pale yellow solid, 4.7 mg, yield = 0.0024%).

Macatanarin D (1): yellow powder; [α]²⁹_D + 33.9 (c 0.05, MeOH); FT-IR (KBr) ν_max: 3414, 2928, 1725, 1656, 1610, 1475, 1374 1163, 1087, 967, 894, 745, 647; UV (MeOH) λ_max (log ε): 210 (2.6), 271 (1.25), 327 (1.15), 366 (1.20); ¹H and ¹³C-NMR spectral data (see Table 1); HR-ESI MS: m/z 481.1864 [M+H]⁺ (calcd. for C₂₇H₂₉O₈, 481.1862).

2.4. Cytotoxic Assay

The cytotoxicity assays were carried out in triplicate in 96-well microtiter plates against KB cell line (mouth epidermal carcinoma cells) and MCF-7 cell line (breast cancer cells). Cells were maintained in Dulbecco’s DMEM medium, supplemented with 10% fetal calf serum, L-glutamine (2 mM), penicillin G (100 UI/mL), streptomycin (100 μg/mL), and gentamicin (10 μg/mL). Stock solutions of compounds were prepared in DMSO/H₂O (1/9), and the cytotoxicity assays were carried out in 96-well microtiter plates against cancer or normal cells (3 × 10³ cells/mL) using a modification of the published method [14]. After 72 h incubation at 37°C in air/CO₂ (95 : 5) with or without test compounds, cell growth was estimated by colorimetric measurement of living cells stained by neutral red. Optical density was determined at 540 nm with a Titertek Multiskan photometer. The IC₅₀ value was defined as the concentration of the sample necessary to inhibit the cell growth to 50% of the control. Ellipticine was used as a reference compound.

3. Results and Discussion

Compound 1 was isolated as a yellow powder, and its molecular formula of C₂₇H₂₈O₈ was established by HRESIMS at m/z 481.1864 [M+H]⁺ (calcd. for C₂₇H₂₉O₈, 481.1862). The FT-IR showed absorption bands at 3414, 1657, 1610, and 1475 cm⁻¹ indicating the presence of hydroxy, α, β-unsaturated carbonyl and aromatic ring functionalities, respectively. The UV absorption maximum at 366, 327, and 271 nm was typical for a flavonol-type compound [15]. The presence of a substituted flavonol skeleton was suggested by the analysis of ¹H and ¹³C-NMR spectroscopic data (Table 1). The NMR spectroscopic data of 1 were similar to those of macakurzin B, which has been previously isolated from M. kurzii, except for the presence of a prenyl, acetyl, and OH groups [6]. In the ¹H-NMR spectrum, the presence of an ABX system at δ_H 6.92 (d, J = 8.5 Hz), 7.80 (dd, J = 2.5, 8.5 Hz), and 7.83 (d, J = 2.5 Hz) and a singlet proton at δ_H 6.45 was observed in the aromatic region. Additionally, the ¹H-NMR data also exhibited an acetyl group at δ_H 1.88 (3H, s), and two isoprenoid units: a 3-methyl-2-butenyl group (δ_H 1.71 and 1.72 (each 3H, s), 5.31 (1H, t, J = 7.5 Hz), and 3.28 (2H, d, J = 7.5 Hz)), and a 2,2-dimethyl-3-hydroxy-dihydropyrano ring (δ_H 1.20 and 1.34 (each 3H, s), 3.67 (1H, dd, J = 5.5, 7.5 Hz), 2.77 (1H, dd, J = 5.5, 17.0 Hz), and 2.40 (1H, dd, J = 7.5, 17.0 Hz)). The analysis of ¹³C-NMR data and 2D HSQC spectrum of 1 revealed the presence of 27 carbons, corresponding to a flavonol derivative with one acetyl group and two isoprene moieties (Table 1).

The HMBC correlations of H-1‴ (δ_H 3.28) with C-2′ (δ_C 128.2), C-3′ (δ_C 127.5), C-4′ (δ_C 156.1), C-2‴ (δ_C 122.6), C-3‴ (δ_C 131.6) and OH (δ_H 7.29) with C-4′ (δ_C 156.1), C-5′ (δ_C 114.9), and C-3′ (δ_C 127.5) determined the linkage of the isoprenyl chain with C-3′ and position of OH group at C-4′ on ring B (Figure 1). Furthermore, the COSY correlations of CH₂-4″/H-5″ and the HMBC cross peaks of H-4″ (δ_H 2.40 and 2.77) with C-5″/C-6″/C-6/C-5/C-7 and OH (δ_H 3.09) with C-4″ (δ_C 26.0), C-5″ (δ_C 67.1), and C-6″ (δ_C 77.5) were confirmed that the position of OH group was at C-5″ of pyrano ring and 2,2-dimethyldihydropyranol moiety was attached to ring A. From the ¹H-NMR spectrum, absence of resonance for a hydrogen-bonded hydroxy proton, led to the assumption that a free hydroxy group was not present at C-5. Two possible isomers could be considered at this stage. In the first one, the dihydropyranol moiety is fused to ring A via C-5 and C-6 and the O-acetyl group is located at C-7, while in the second possible isomer, the pyranol moiety is fused to ring A via C-6 and C-7 and the O-acetyl group is attached in position C-5. Since only the correlations of the methyl protons of acetyl group at δ_H 1.88 with methylene protons of CH₂-4″ at δ_H 2.40 and 2.77 and no NOE interaction between the aromatic singlet H-8 (δ_H 6.45) and the methyl protons of acetyl group were observed on the NOESY spectrum, the pyranol moiety is supposed to be attached at C-6 and C-7 and the acetyl group in C-5 on ring A. This suggestion agreed with NMR data found for structurally close prenylated flavonoids. In particular, when a pyrane moiety is fused via C6 and C-7 on ring A, the resonance of C-7 is around δ_C 161.0–164.0 (δ_C 161.2 for dinklagin B [16] and 163.7 for tanariflavanone B [17], δ_C 161.1 for compound 1), whereas a pyrane moiety fused via C5 and C-6 leads to a upfield-shifted carbon C-5 signal at about 155.0 (δ_C 155.5 and 155.7 for vogelins I and J, respectively [18]).

The relative configuration of C-5″ was established by proton coupling constant analysis and NOESY spectrum. The pseudoaxial orientation of H-5″ can be deduced from its proton coupling constants with a gauche (J = 5.5 Hz) and an anti (7.5 Hz). This observation was confirmed by the NOESY data analysis, which showed NOE correlations between H-5″ (δ_H 3.67) and CH₃-7″ (δ_H 1.34) and proton H_b-4″ (δ_H 2.77) of the 4″-CH₂ group. Based on these observations, the structure of the newly isolated compound 1 was determined as 5-O-acetyl-6,7-(2,2-dimethyl-3-hydroxydihydropyrano)-3′-prenyl kaempferol (Figure 2) and named “macatanarin D.”

The structures of the known stilbenes: schweinfurthin H (2) [19], vedelianin (3) [20], schweinfurthin F (4), schweinfurthin E (5) [19], and 4′deprenyl-mappain (6) [21] were determined by analysis of spectroscopic data and comparison with reported data. So far, about 90% of the isolated compounds come from the leaves of Macaranga genus while 10% were isolated from other plant parts such stem and root barks, fruits, seeds, and flowers. No phytochemical studies had been conducted to date on glandular trichomes of Macaranga fruits. It is important to note that collecting time clearly influences the harvesting yield of glandular trichomes. While the young fruits do not have glandular trichomes and overripe fruits contain low yield of glandular trichomes, the adult/mature fruits, with clearly visible trichome glands, give the best results. In Vietnam, it is best to harvest mature fruits in June.

Since prenylated stilbenes and flavonoids of Macaranga genus are reported to have potent cytotoxic activities [9, 22], compounds 1–6 were evaluated for their cytotoxic activity against KB and MCF-7 human cancer cell lines. Ellipticine was used as a reference compound. The results are shown in Table 2. Compounds 1 and 6 showed moderate cytotoxic activities against KB cell line with IC₅₀ values of 29.3 and 24.7 μM, respectively. Compounds 3, 4, and 5 showed the strongest activities against both KB and MCF-7 cell lines with IC₅₀ values in the range of 0.03–0.12 μM, which is evenly stronger than ellipticine. It was worth noting that three aforementioned compounds possessed the same hexahydroxanthene moiety but a variable number of hydroxy groups, which may explain the difference in their cytotoxic potencies. Compound 2 also showed a significant cytotoxicity against KB cell line (IC₅₀ = 0.26) but compared to compounds 3, 4, and 5, cytotoxicity appears to be much less active against the MCF-7 cell line with an IC₅₀ value of 10.4.

4. Conclusion

An undescribed flavonoid, macatanarin D (1), together with five known prenylated stilbenes (2–6) were isolated from glandular trichomes of fruits of Macaranga tanarius. Most of the compounds isolated have shown potent cytotoxic activities against the two cancer cell lines KB and MCF-7. It is postulated that these specialized metabolites are an important first line of defense against herbivorous insects and/or pathogens.

Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that there are no conflicts of interest regarding the publication of this paper.

Acknowledgments

This work was financially supported by the Vietnam Academy of Science and Technology (QTFR01.01/18-19) and the framework of International Associated Laboratory (LIA “NATPROCHEMLAB”) between the Centre National de la Recherche Scientifique (CNRS, ICSN, France) and the Vietnam Academy of Science and Technology (Institute of Marine Biochemistry, VAST, Vietnam). Additional research support was provided through the Ministry of Science and Technology of Vietnam (Grant number ĐTĐLCN.14/16).

Supplementary Materials

Figure 1S: HR-ESI-MS of 1. Figure 2S: ¹H NMR spectrum of 1. Figure 3S: ¹³C NMR spectrum of 1. Figure 4S: HSQC spectrum of 1. Figure 5S: HMBC spectrum of 1. Figure 6S: COSY spectrum of 1. Figure 7S: NOESY spectrum of 1. ¹H and ¹³C NMR spectroscopic data of isolated compounds 2–6. (Supplementary Materials)

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Copyright © 2019 Thi Mai Huong Doan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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