TY - JOUR A2 - Ruberto, Giuseppe AU - Le, Dinh Chi AU - Ngo, Thi Duyen AU - Hoa Le, Thi Huong PY - 2019 DA - 2019/11/29 TI - Simultaneous Assay of Dexchlorpheniramine Maleate, Betamethasone, and Sodium Benzoate in Syrup by a Reliable and Robust HPLC Method SP - 2952075 VL - 2019 AB - The simultaneous determination of betamethasone, dexchlorpheniramine maleate, and sodium benzoate in pharmaceutical syrup was done by using a simple validated HPLC method. The chromatographic separation of the three analytes was done in a C18 column maintained at 25°C, using a mixture of acetonitrile and 0.02 M phosphate buffer solution pH 2.70 (35 : 65, v : v) as mobile phase. The isocratic elution was chosen with total flow rate of mobile phase maintained at 1.0 mL per minute. The analytes were detected by a UV-Vis detector set at 254 nm. Injection volume was set at 50 μl. The method was fully validated in terms of specificity, linearity, precision, accuracy, and robustness according to requirements of current guidelines and was proven to be suitable for the intended application. SN - 2090-8865 UR - https://doi.org/10.1155/2019/2952075 DO - 10.1155/2019/2952075 JF - Journal of Analytical Methods in Chemistry PB - Hindawi KW - ER -