Simultaneous Analysis of Twelve Bile Acids by UPLC-MS and Exploration of the Processing Mechanism of Bile Arisaema by Fermentation

Table 2

The optimized MRM parameters and transitions for each analyst in UPLC-QqQ-MS/MS.

Analyte	tR (min)	[M-H]^−(m/z)	MRM transitions (precursor ⟶ product)	Cone voltage (V)	Collision energy (eV)
(1) THCA	1.25	514.39	514.39 ⟶ 80.14	100.0	66.0
(2) THDCA	1.60	498.39	498.39 ⟶ 80.14	100.0	65.0
(3) TCA	1.74	514.39	514.39 ⟶ 80.14	100.0	66.0
(4) GHCA	2.20	464.40	464.40 ⟶ 74.10	76.0	38.0
(5) GCA	2.74	464.40	464.40 ⟶ 74.10	76.0	38.0
(6) GHDCA	2.77	448.41	448.41 ⟶ 74.10	74.0	36.0
(7) TCDCA	2.83	498.39	498.39 ⟶ 80.14	100.0	65.0
(8) HCA	3.55	407.31	407.31 ⟶ 343.42	78.0	30.0
(9) CA	4.12	407.31	407.31 ⟶ 343.42	78.0	30.0
(10) GCDCA	4.41	448.41	448.41 ⟶ 74.10	74.0	36.0
(11) HDCA	4.47	391.35	391.35 ⟶ 345.49	80.0	34.0
(12) CDCA	8.04	391.35	391.35 ⟶ 345.49	80.0	34.0
(13) Ginsenoside Rh1	1.05	637.43	637.43 ⟶ 475.26	100.0	40.0