Preliminary Studies on Liquiritin, Deoxyschizandrin, and Tanshinone II A as Potential Anti-Neurodegenerative Disease Agent: Determination by Reverse-Phase Liquid Chromatography in Tianwang Buxin Pills

Table 1

Optimization of gradient conditions.

Chromatographic factor	Conditions	Optimized condition	Reason
Elution gradient	(1) 0–10 min, 0–15% B; 10–30 min, 15–30% B; 30–70 min, 30–75%; 70–85 min, 75% B; 85–86 min, 75–0% B; 86–100 min, 0% B;	Procedure (1)	High peak resolution
	(2) 0–10 min, 0–15% B; 10–30 min, 15–30% B; 30–60 min, 30–65%; 60–70 min, 65–70% B; 70–85 min, 70–75% B; 85–86 min, 75–0% B, 86–100 min, 0% B