Comparison of Phenolic Compound Separations by HPTLC and PPEC with SDS as the Mobile Phase Component

Figure 1

Migration distance of flavonoids vs. SDS concentration in the mobile phase for HPTLC (a) and PPEC (b) experiments. The mobile phase: butanol (0.89 M; 8.14% v/v), 10% acetonitrile, and SDS in the range of 0.0143 to 0.1144 M aqueous buffer of pH 7.0 (0.88 mM of citric acid and 8.23 mM of disodium hydrogen phosphate). The stationary phase: HPTLC RP-18W. TLC: experiment time 20 min. PPEC: polarization voltage 800 V; experiment time 8 min.

(a)

(b)