TY - JOUR AU - Tang, Ying AU - Jia, Changxin AU - He, Jianshuai AU - Zhao, Yang AU - Chen, Huayong AU - Wang, Shilei PY - 2019 DA - 2019/12/27 TI - The Application and Analytical Pathway of Dexmedetomidine in Ischemia/Reperfusion Injury SP - 7158142 VL - 2019 AB - Ischemia/reperfusion cerebral injury can cause serious damage to nerve cells. The injured organelles are cleared by autophagy eventually, which is critical for cell survival. Dexmedetomidine is neuroprotective in various ischemia/reperfusion models. Mitochondrial calcium uniporter (MCU) is the most important channel of mitochondrial Ca2+ influx into mitochondria, where Ca2+ has a potential effect on mitochondrial autophagy. However, the role of MCU in the changes of mitophagy and autophagy caused by dexmedetomidine is unknown. In this study, we constructed an in vitro I/R model by subjecting the oxygen and glucose deprivation/reperfusion model to SH-SY5Y cells to mimic the cerebral I/R injury. We found that postconditioning with dexmedetomidine and 3-methyladenine (3MA, an autophagy inhibitor) increased the cell survival meanwhile reduced the production of autophagic vesicles and the expression of LC3 and Beclin 1. This process also increased the expression of BCL-2, P62, and TOM20. After applied with spermine (MCU-specific agonist), the expression of autophagy proteins by dexmedetomidine was reversed, and the same changes were also observed in immunofluorescence. The results of our study suggested that dexmedetomidine can inhibit MCU and reduce excessive mitophagy and autophagy for conferring protection against I/R injury. SN - 2090-8865 UR - https://doi.org/10.1155/2019/7158142 DO - 10.1155/2019/7158142 JF - Journal of Analytical Methods in Chemistry PB - Hindawi KW - ER -