Quantitative Analysis of Eight Triterpenoids and Two Sesquiterpenoids in Rhizoma Alismatis by Using UPLC-ESI/APCI-MS/MS and Its Application to Optimisation of Best Harvest Time and Crude Processing Temperature

Table 1

Retention time, related MS data of the 10 investigated compounds, and internal standards in the UPLC-APCI/ESI-MS/MS analysis.

Compounds	tR (min)	Fragment ions (m/z)	Cone voltage (V)	Collision energy (eV)	Ionisation modes
Alismoxide	1.48	203.1  ⟶  161.0; 203.1  ⟶  147.0; 203.1  ⟶  133.0; 203.1  ⟶  105.01; 203.1  ⟶  95.01	15	15	APCI+
Alisol C	2.05	487.1  ⟶  469.1; 487.1 ⟶ 451.1; 487.1 ⟶ 415.1; 487.1 ⟶ 379.1; 469.1 ⟶ 451.1; 469.1 ⟶ 415.1; 469.1 ⟶ 379.1	35	15	ESI+
Alisol C 23-acetate	2.57	529.3 ⟶ 511.3; 529.3 ⟶ 469.3; 529.3 ⟶ 451.3; 529.3 ⟶ 415.3; 529.3 ⟶ 397.3	30	20	ESI+
Alisol A	3.03	473.3 ⟶ 337.0; 473.3 ⟶ 365.3; 473.3 ⟶ 383.3; 473.3 ⟶ 437.3; 473.3 ⟶ 455.3	40	11	ESI+
Alisol A 24-acetate	3.5	515.3 ⟶ 497.3; 515.3 ⟶ 437.3; 515.3 ⟶ 455.3; 515.3 ⟶ 383.3; 515.3 ⟶ 365.3	20	10	ESI+
Alismol	3.64	203.1 ⟶ 161.0; 203.1 ⟶ 147.0; 203.1 ⟶ 133.0; 203.1 ⟶ 109.0; 203.1 ⟶ 95.01	10	12	APCI+
Alisol B	4.23	455.1 ⟶ 437.0; 455.1 ⟶ 383.1; 455.1 ⟶ 379.1; 383.3 ⟶ 365.3	40	27	ESI+
Alisol B 23-acetate	4.99	497.1 ⟶ 479.1; 497.1 ⟶ 437.2; 497.1 ⟶ 419.1; 497.1 ⟶ 383.1; 497.1 ⟶ 365.1	40	8	ESI+
11-Deoxyalisol B	5.81	457.1 ⟶ 439.1; 457.1 ⟶ 385.1; 385.3 ⟶ 367.7	40	10	ESI+
11-Deoxyalisol B 23-acetate	6.68	499.2 ⟶ 439.3; 499.2 ⟶ 385.3; 499.2 ⟶ 421.0; 383.1 ⟶ 367.7	35	12	ESI+
Glycyrrhetnic acid (IS)	3.76	417.3 ⟶ 317.2	35	12	ESI+

^∗Quantitative ion pair.