Fingerprint Spectrophotometric Methods for the Determination of Co-Formulated Otic Solution of Ciprofloxacin and Fluocinolone Acetonide in Their Challengeable Ratio

Table 3

Analysis of laboratory-prepared mixtures and the dosage form by the proposed spectrophotometric methods.

CIP : FLU^a (μg/mL)	CIP						FLU
	Recovery %
	a-Centering		EXRS	CM	UCS	SS	a-Centering		RS
	NS	FS					NS	FS
5 : 5	98.00	99.80	101.78	99.80	102.04	100.60	97.80	99.80	100.06
10 : 4	99.90	100.10	99.80	101.09	100.59	99.90	100.05	100.05	100.00
4 : 10	100.05	100.03	100.15	99.75	100.75	98.58	99.90	99.80	100.02
4 : 8	100.15	101.78	100.08	100.08	99.75	101.28	99.84	100.01	98.63
8 : 4	99.88	100.04	99.88	98.75	101.25	100.38	99.75	99.50	100.05
12 : 5^b	100.03	99.99	100.04	100.05	100.03	100.00	100.06	99.80	99.80
Mean^c ± SD	99.67 ± 0.82	100.29 ± 0.74	100.29 ± 0.74	99.92 ± 0.75	100.73 ± 0.83	100.12 ± 0.90	99.57 ± 0.87	99.83 ± 0.20	99.76 ± 0.56
^a,dOtovel® batch no. 24338-080-14	100.03 ± 0.56	99.81 ± 0.36	99.66 ± 0.54	99.44 ± 0.61	99.61 ± 0.52	99.75 ± 0.78	100.27 ± 1.20	99.45 ± 1.31	99.37 ± 1.21

^aAverage of three experiments. ^bRatio present in Otovel® before subtraction of the added (FLU) spectrum (4 μg/mL). ^cMean of the percentage recovery of all laboratory-prepared mixtures and standard deviation. ^dMean and standard deviation of the percentage recovery of Otovel®. NS results were obtained by using the normalized spectrum. FS results were obtained by using the factorized spectrum.