Research Article

Optimization of Extraction Conditions for Determination of Tiliroside in Tilia L. Flowers Using an LC-ESI-MS/MS Method

Table 8

Total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity by the DPPH˙ method, and free radical scavenging ability (ABTS+˙) in different extracts (70% ethanol with addition of 1% acetic acid) from Tilia L. flowers.

SampleTPC (mg·GA·g−1 of dry extract)TFC (mg·Q·g−1 of dry extract)TEAC (mM·Trolox·g−1 dry extract)IC50 (mg·mg−1·DPPH˙)

MACst-E70% + AA1%239.09 ± 15.6623.70 ± 0.891.06 ± 0.030.25 ± 0.01
ASE-E70% (80) + AA1%296.96 ± 15.6616.89 ± 0.861.54 ± 0.050.30 ± 0.02
UAE-E70% (80) + AA1%238.69 ± 15.4620.58 ± 0.081.54 ± 0.020.25 ± 0.01
UR-E70% + AA1%181.39 ± 2.6214.05 ± 0.711.17 ± 0.010.34 ± 0.01