Research Article

A Sensitive and Efficient Method for Determination of Capecitabine and Its Five Metabolites in Human Plasma Based on One-Step Liquid-Liquid Extraction

Table 4

Inter- and intraprecision and accuracy of Cap and its 5 metabolites (n = 5).

AnalyteNominal Concentration (ng/mL)IntradayInterday
Measured concentration(ng/mL, mean ± SD)Precision (RSD%)Accuracy (RE%)Measured concentration (ng/mL, mean ± SD)Precision (RSD%)Accuracy (RE%)

Cap20.4019.70 ± 1.548.24−3.4019.30 ± 1.588.04−5.70
51.0048.61 ± 4.9110.09−4.7950.07 ± 2.785.20−2.15
1020.00924.82 ± 58.806.36−9.33991.58 ± 86.258.33−3.13
2550.002535 ± 37.931.50−0.562306.09 ± 160.756.69−9.88

5’-DFCR20.0022.82 ± 0.441.9414.1321.78 ± 1.014.658.88
50.0046.77 ± 0.621.33−6.4646.92 ± 1.392.97−6.15
1000.00977.35 ± 17.151.75−2.271014.99 ± 48.904.821.50
2500.002614.20 ± 23.700.914.572553.80 ± 74.242.912.15

5’-DFUR20.4023.52 ± 0.883.7515.2922.74 ± 1.256.168.53
51.0053.51 ± 1.693.164.9152.35 ± 2.134.530.11
1020.001011.02 ± 20.722.05−0.891108.28 ± 7.054.835.82
2550.002625.62 ± 25.970.992.972642.50 ± 154.563.060.94

2’-DFUR22.0023.20 ± 2.3610.195.4521.93 ± 2.376.270.59
55.0055.53 ± 2.163.890.9652.80 ± 3.834.45−1.11
1100.001100.18 ± 36.893.350.021134.77 ± 57.844.485.77
2750.002741.87 ± 46.131.68−0.292635.74 ± 88.382.69−1.73

5-FU20.6019.94 ± 1.356.77−3.3119.50 ± 1.628.30−5.52
51.5052.91 ± 1.212.282.7352.91 ± 2.554.812.73
1030.001082.02 ± 22.352.065.051094.09 ± 22.642.076.22
2575.002443.83 ± 14.000.57−5.102432.66 ± 28.221.16−5.53

FUH220.2022.90 ± 1.456.3413.3921.15 ± 2.2110.454.69
50.5048.46 ± 4.098.43−4.0547.93 ± 3.356.99−5.10
1010.00936.66 ± 23.462.50−7.26961.43 ± 38.604.01−4.81
2525.002521.59 ± 2.682.68−0.132579.34 ± 106.564.132.15