Research Article
A Sensitive and Efficient Method for Determination of Capecitabine and Its Five Metabolites in Human Plasma Based on One-Step Liquid-Liquid Extraction
Table 4
Inter- and intraprecision and accuracy of Cap and its 5 metabolites (n = 5).
| Analyte | Nominal Concentration (ng/mL) | Intraday | Interday | Measured concentration(ng/mL, mean ± SD) | Precision (RSD%) | Accuracy (RE%) | Measured concentration (ng/mL, mean ± SD) | Precision (RSD%) | Accuracy (RE%) |
| Cap | 20.40 | 19.70 ± 1.54 | 8.24 | −3.40 | 19.30 ± 1.58 | 8.04 | −5.70 | 51.00 | 48.61 ± 4.91 | 10.09 | −4.79 | 50.07 ± 2.78 | 5.20 | −2.15 | 1020.00 | 924.82 ± 58.80 | 6.36 | −9.33 | 991.58 ± 86.25 | 8.33 | −3.13 | 2550.00 | 2535 ± 37.93 | 1.50 | −0.56 | 2306.09 ± 160.75 | 6.69 | −9.88 |
| 5’-DFCR | 20.00 | 22.82 ± 0.44 | 1.94 | 14.13 | 21.78 ± 1.01 | 4.65 | 8.88 | 50.00 | 46.77 ± 0.62 | 1.33 | −6.46 | 46.92 ± 1.39 | 2.97 | −6.15 | 1000.00 | 977.35 ± 17.15 | 1.75 | −2.27 | 1014.99 ± 48.90 | 4.82 | 1.50 | 2500.00 | 2614.20 ± 23.70 | 0.91 | 4.57 | 2553.80 ± 74.24 | 2.91 | 2.15 |
| 5’-DFUR | 20.40 | 23.52 ± 0.88 | 3.75 | 15.29 | 22.74 ± 1.25 | 6.16 | 8.53 | 51.00 | 53.51 ± 1.69 | 3.16 | 4.91 | 52.35 ± 2.13 | 4.53 | 0.11 | 1020.00 | 1011.02 ± 20.72 | 2.05 | −0.89 | 1108.28 ± 7.05 | 4.83 | 5.82 | 2550.00 | 2625.62 ± 25.97 | 0.99 | 2.97 | 2642.50 ± 154.56 | 3.06 | 0.94 |
| 2’-DFUR | 22.00 | 23.20 ± 2.36 | 10.19 | 5.45 | 21.93 ± 2.37 | 6.27 | 0.59 | 55.00 | 55.53 ± 2.16 | 3.89 | 0.96 | 52.80 ± 3.83 | 4.45 | −1.11 | 1100.00 | 1100.18 ± 36.89 | 3.35 | 0.02 | 1134.77 ± 57.84 | 4.48 | 5.77 | 2750.00 | 2741.87 ± 46.13 | 1.68 | −0.29 | 2635.74 ± 88.38 | 2.69 | −1.73 |
| 5-FU | 20.60 | 19.94 ± 1.35 | 6.77 | −3.31 | 19.50 ± 1.62 | 8.30 | −5.52 | 51.50 | 52.91 ± 1.21 | 2.28 | 2.73 | 52.91 ± 2.55 | 4.81 | 2.73 | 1030.00 | 1082.02 ± 22.35 | 2.06 | 5.05 | 1094.09 ± 22.64 | 2.07 | 6.22 | 2575.00 | 2443.83 ± 14.00 | 0.57 | −5.10 | 2432.66 ± 28.22 | 1.16 | −5.53 |
| FUH2 | 20.20 | 22.90 ± 1.45 | 6.34 | 13.39 | 21.15 ± 2.21 | 10.45 | 4.69 | 50.50 | 48.46 ± 4.09 | 8.43 | −4.05 | 47.93 ± 3.35 | 6.99 | −5.10 | 1010.00 | 936.66 ± 23.46 | 2.50 | −7.26 | 961.43 ± 38.60 | 4.01 | −4.81 | 2525.00 | 2521.59 ± 2.68 | 2.68 | −0.13 | 2579.34 ± 106.56 | 4.13 | 2.15 |
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