Determination of Isosorbide-5-Mononitrate in Human Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry and Its Application to a Bioequivalence Study

Table 3

Extraction recovery and matrix effect of 5-ISMN in plasma (n = 6).

Compound	Nominal con. (ng/mL)	Recovery (%) n = 6	Mean recovery% (RSD %)	Matrix effect (%) n = 6		IS-normalized matrix effect	Mean matrix effect% (RSD %)
				5-ISMN	IS
5-ISMN	8	82.6	87.0 (7.0)	95.5	88.6	107.8	102.1 (0.85)
	80	84.3		87.0	88.3	98.6
^13C6-5-ISMN	800	93.8		93.4	94.3	99.8
	1000	80.9	NA	NA	NA	NA	102.1 (0.67)

Concentration is in 3 significant figures; RSD%, percent relative standard deviation (in one decimal place). NA, not available.