Journal of Analytical Methods in Chemistry

Research Article

Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats

Table 1

The precursor ions, product ions, declustering potential (DP), and collision energy (CE) for each analyte and internal standard (IS) in negative and positive ionization modes.


Compounds	Molecular weight	Ionization mode	Monitoring ion	Parent ion	Daughter ion	DP (V)	CE (V)

Rhein (IS)	284.22	ESI⁻	[M − H]⁻	283.2	238.9	−59.15	−19.01
Geniposidic acid	374.34	ESI⁻	[M − H]⁻	373.0	211.3	−126.4	−18.65
Psoralidin	336.34	ESI⁻	[M − H]⁻	335.0	280.0	−127.91	−35.4
Neobavaisoflavone	322.37	ESI⁻	[M − H]⁻	321.0	264.8	−113.91	−38.92
Bavachinin	338.40	ESI⁻	[M − H]⁻	337.1	119.0	−59.91	−27.28
Escoparone (IS)	206.19	ESI⁺	[M + H]⁺	207.2	151.1	93.08	27.23
Psoralenoside	366.32	ESI⁺	[M + H]⁺	187.2	131.0	82.75	33.1
Psoralen	186.16	ESI⁺	[M + H]⁺	187.2	131.0	82.75	33.1
Isopsoralen	186.16	ESI⁺	[M + H]⁺	187.2	131.0	82.75	33.1
Bavachin	324.37	ESI⁺	[M + H]⁺	325.4	149.0	79.14	30.73
Isobavachin	324.37	ESI⁺	[M + H]⁺	325.4	149.0	79.14	30.73
Bavachalcone	324.37	ESI⁺	[M + H]⁺	325.4	149.0	79.14	30.73
Isobavachalcone	324.37	ESI⁺	[M + H]⁺	325.4	149.0	79.14	30.73
Pinoresinol diglucoside	682.67	ESI⁺	[M + H₂O]⁺	700.5	234.9	86.44	19.14