Journal of Analytical Methods in Chemistry / 2020 / Article / Tab 2

Research Article

Establishment of a UPLC-MS/MS Method for Studying the Effect of Salt-Processing on Tissue Distribution of Twelve Major Bioactive Components of Qing’e Pills in Rats

Table 2

Calibration curves, correlation coefficient, linear range, and LLOQ of 12 compounds.

CompoundTissueLinear regression equationCorrelation coefficient (r)Linear range (ng/mL)LLOQ (ng/mL)

PsoralenHeartY = 7.77 × 10−3X + 2.23 × 10−21.00001–10001.0
LiverY = 3.60 × 10−3X + 1.03 × 10−11.00005–10005.0
SpleenY = 5.71 × 10−3X + 2.92 × 10−20.99955–10005.0
LungY = 2.44 × 10−3X + 3.33 × 10−20.99805–10005.0
KidneyY = 3.44 × 10−3X + 1.21 × 10−10.99962–10002.0
UterusY = 8.62 × 10−3X + 8.86 × 10−31.00001–10001.0
OvaryY = 6.53 × 10−3X + 1.79 × 10−21.00005–10005.0
TestesY = 5.04 × 10−3X + 4.89 × 10−21.00001–10001.0

IsopsoralenHeartY = 8.57 × 10−3X + 3.61 × 10−21.00001–10001.0
LiverY = 1.65 × 10−3X + 1.74 × 10−10.99865–10005.0
SpleenY = 4.55 × 10−3X + 3.50 × 10−20.99815–10005.0
LungY = 1.48 × 10−3X + 5.62 × 10−20.99705–10005.0
KidneyY = 2.34 × 10−3X + 1.77 × 10−10.99942–10002.0
UterusY = 6.23 × 10−3X + 2.86 × 10−21.00001–5001.0
OvaryY = 6.12 × 10−3X + 5.19 × 10−21.00005–10005.0
TestesY = 4.27 × 10−3X + 7.24 × 10−20.99975–10005.0

BavachinHeartY = 2.31 × 10−2X − 6.13 × 10−31.00000.4–500.4
LiverY = 3.17 × 10−2X + 8.99 × 10−20.99771–5001.0
SpleenY = 2.61 × 10−2X + 3.94 × 10−21.00001–2001.0
LungY = 4.07 × 10−2X + 2.90 × 10−20.99760.5–2000.5
KidneyY = 2.19 × 10−2X + 6.66 × 10−30.99991–5001.0
UterusY = 2.11 × 10−2X − 4.30 × 10−20.99971–2001.0
OvaryY = 3.70 × 10−2X − 1.14 × 10−21.00001–501.0
TestesY = 2.14 × 10−2X − 2.71 × 10−20.98971–501.0

IsobavachinHeartY = 4.07 × 10−2X + 8.55 × 10−30.99960.3–500.3
LiverY = 5.35 × 10−2X + 5.28 × 10−20.99991–2501.0
SpleenY = 3.82 × 10−2X + 8.21 × 10−20.99950.5–1000.5
LungY = 5.94 × 10−2X + 3.32 × 10−20.99760.5–1000.5
KidneyY = 4.62 × 10−2X + 1.85 × 10−21.00000.1–2500.1
UterusY = 3.93 × 10−2X + 3.14 × 10−31.00000.1–500.1
OvaryY = 7.17 × 10−2X − 7.18 × 10−20.99991–501.0
TestesY = 3.94 × 10−2X + 7.50 × 10−20.98870.5–500.5

Pinoresinol diglucosideHeartY = 6.50 × 10−4X − 2.98 × 10−41.00000.5–1000.5
LiverY = 8.57 × 10−4X + 6.87 × 10−50.99990.5–2500.5
SpleenY = 4.46 × 10−4X + 5.35 × 10−41.00000.5–1000.5
LungY = 6.95 × 10−4X + 1.26 × 10−31.00000.5–5000.5
KidneyY = 3.45 × 10−4X + 5.16 × 10−40.99981–10001.0
UterusY = 6.24 × 10−4X + 6.35 × 10−40.99960.5–1000.5
OvaryY = 7.81 × 10−4X − 3.66 × 10−40.99970.5–1000.5
TestesY = 6.90 × 10−4X + 6.48 × 10−40.99990.5–500.5

PsoralenosideHeartY = 6.99 × 10−4X + 2.09 × 10−30.99935–10005.0
LiverY = 4.63 × 10−4X + 1.21 × 10−20.99965–10005.0
SpleenY = 5.56 × 10−4X + 2.73 × 10−30.99755–10005.0
LungY = 9.26 × 10−4X + 2.96 × 10−20.99981–10001.0
KidneyY = 3.61 × 10−4X + 8.94 × 10−30.99915–20005.0
UterusY = 9.86 × 10−4X + 3.82 × 10−20.99945–20005.0
OvaryY = 6.00 × 10−4X + 1.93 × 10−20.99985–20005.0
TestesY = 5.16 × 10−4X + 1.57 × 10−20.99735–10005.0

IsobavachalconeHeartY = 4.89 × 10−2X − 5.05 × 10−20.99971–501.0
LiverY = 8.17 × 10−2X + 9.05 × 10−20.99731–1001.0
SpleenY = 4.86 × 10−2X + 6.15 × 10−20.99980.5–2500.5
LungY = 4.93 × 10−2X + 1.59 × 10−30.99980.5–2500.5
KidneyY = 3.18 × 10−2X + 2.75 × 10−20.99931–5001.0
UterusY = 3.47 × 10−2X − 4.89 × 10−20.99951–501.0
OvaryY = 2.29 × 10−2X + 1.08 × 10−20.99990.5–500.5
TestesY = 2.88 × 10−2X + 2.11 × 10−21.00000.5–500.5

BavachalconeHeartY = 2.47 × 10−2X − 8.09 × 10−30.99670.3–250.3
LiverY = 2.83 × 10−2X + 1.88 × 10−20.99990.5–2500.5
SpleenY = 2.83 × 10−2X + 2.61 × 10−20.99960.5–500.5
LungY = 3.05 × 10−2X + 3.49 × 10−20.99970.5–1000.5
KidneyY = 3.01 × 10−2X + 2.78 × 10−20.99920.5–1000.5
UterusY = 3.25 × 10−2X − 2.93 × 10−20.99980.5–2500.5
OvaryY = 2.17 × 10−2X + 1.12 × 10−21.00000.5–500.5
TestesY = 2.63 × 10−2X − 1.85 × 10−20.99960.5–500.5

Geniposidic acidHeartY = 1.11 × 10−3X + 4.13 × 10−40.99961–5001.0
LiverY = 6.88 × 10−4X + 3.58 × 10−30.99931–10001.0
SpleenY = 1.31 × 10−3X + 1.03 × 10−30.99901–5001.0
LungY = 8.89 × 10−4X + 1.78 × 10−30.99861–10001.0
KidneyY = 8.11 × 10−4 X - 1.72 × 10−30.99921–20001.0
UterusY = 5.08 × 10−3X + 1.48 × 10−20.99950.5–5000.5
OvaryY = 8.04 × 10−4X + 7.50 × 10−40.99901–10001.0
TestesY = 5.83 × 10−4X + 3.28 × 10−40.99561–2501.0

PsoralidinHeartY = 9.87 × 10−3X − 2.03 × 10−30.99991–2501.0
LiverY = 7.80 × 10−3X + 1.11 × 10−20.99971–5001.0
SpleenY = 2.43 × 10−2X − 2.60 × 10−30.99981–5001.0
LungY = 1.54 × 10−2X + 1.70 × 10−20.99931–5001.0
KidneyY = 6.92 × 10−2X + 3.91 × 10−30.99961–10001.0
UterusY = 3.96 × 10−2X − 6.48 × 10−20.99961–2501.0
OvaryY = 2.11 × 10−2X + 1.84 × 10−20.99990.5–1000.5
TestesY = 1.56 × 10−2X + 1.59 × 10−20.99960.5–500.5

NeobavaisoflavoneHeartY = 1.39 × 10−2X − 5.15 × 10−30.99940.5–500.5
LiverY = 7.88 × 10−3X + 5.31 × 10−20.99911–10001.0
SpleenY = 2.06 × 10−2X + 4.61 × 10−20.99981–5001.0
LungY = 1.43 × 10−2X + 3.16 × 10−21.00001–3001.0
KidneyY = 1.26 × 10−2X − 4.05 × 10−30.99941–10001.0
UterusY = 6.00 × 10−2X − 1.22 × 10−10.99971–501.0
OvaryY = 1.81 × 10−2X + 1.16 × 10−20.99961–1001.0
TestesY = 1.40 × 10−2X + 1.36 × 10−20.99981–501.0

BavachininHeartY = 2.72 × 10−3X − 2.72 × 10−30.99991–1001.0
LiverY = 1.48 × 10−3X + 3.93 × 10−30.99981–10001.0
SpleenY = 7.85 × 10−3X − 2.00 × 10−20.99951–10001.0
LungY = 4.39 × 10−3X − 7.75 × 10−40.99941–5001.0
KidneyY = 3.51 × 10−3X − 3.14 × 10−30.99921–5001.0
UterusY = 1.21 × 10−2X − 1.96 × 10−20.99971–501.0
OvaryY = 6.56 × 10−3X − 9.65 × 10−40.99971–501.0
TestesY = 2.72 × 10−3X − 2.72 × 10−30.99991–1001.0

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.