Research Article
Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum
Table 3
Precision and accuracy of the method at four concentration levels.
| Analytes | QC levels | Referred value (μg/mL) | RSD (%) | Accuracy (%) | Intrarun (n = 6) | Interrun (n = 6×3) | Intrarun (n = 6) | Interrun (n = 6×3) |
| PZA | LLOQ | 1.02 | 8.06 | 8.33 | 98.43 | 100.00 | Low | 1.60 | 4.21 | 3.71 | 94.92 | 95.32 | Medium | 16.0 | 4.59 | 3.46 | 98.38 | 98.46 | High | 48.0 | 2.68 | 3.64 | 99.63 | 100.66 |
| INH | LLOQ | 0.152 | 4.69 | 6.70 | 95.02 | 98.68 | Low | 0.268 | 2.98 | 4.62 | 91.13 | 94.67 | Medium | 2.68 | 4.20 | 4.05 | 97.63 | 99.58 | High | 8.04 | 2.53 | 3.92 | 99.63 | 100.81 |
| EMB | LLOQ | 0.0998 | 5.13 | 5.39 | 97.80 | 100.20 | Low | 0.161 | 2.74 | 3.93 | 91.67 | 94.00 | Medium | 1.61 | 12.46 | 8.49 | 103.82 | 99.58 | High | 4.60 | 3.79 | 3.17 | 104.62 | 104.02 |
| RFP | LLOQ | 0.500 | 5.00 | 7.27 | 90.15 | 98.00 | Low | 0.810 | 4.26 | 5.87 | 94.03 | 99.26 | Medium | 8.100 | 8.73 | 6.43 | 95.70 | 97.77 | High | 24.300 | 3.42 | 3.39 | 96.23 | 95.13 |
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PZA: pyrazinamide, INH: isoniazid, RFP: rifampicin, EMB: ethambutol, QC: quality control, RSD: relative standard derivation, and LLOQ: lower limit of qualification.
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