Research Article
Simultaneous Determination of Phenolic Acids, Anthraquinones, Flavonoids, and Triterpenes of Cynomorii Herba in Different Harvest Times by LC-MS/MS
Table 4
Calibration curves, LOD, LOQ, Repeatability, and Recovery of all analytes (n = 6).
| | Components | Linear regression | Linear range (ng·mL−1) | r 2 | LOD (ng·mL−1) | LOQ (ng·mL−1) | Repeatability RSD (%) | Recovery | | Mean (%) | RSD (%) |
| | Gallic acid | Y = 0.2895X + 0.0354 | 101–5050 | 0.9994 | 5 | 15 | 5.75 | 94.0 | 3.08 | | Protocatechuic acid | Y = 0.1929X − 0.0040 | 20.2–5050 | 0.9994 | 6 | 18 | 4.32 | 95.5 | 4.00 | | Catechin | Y = 0.1018X + 0.0280 | 204–10200 | 0.9998 | 6 | 18 | 5.48 | 101.6 | 6.43 | | Protocatechuic aldehyde | Y = 0.0122X + 0.0034 | 44.8–1120 | 0.9998 | 1.5 | 4 | 3.71 | 100.5 | 5.04 | | Epicatechin | Y = 0.9618X + 0.0280 | 21.6–1080 | 0.9998 | 7 | 21 | 3.11 | 97.7 | 5.12 | | Ferulic acid | Y = 0.1414X − 2.2003e-004 | 20.2–1020 | 0.9995 | 3 | 10 | 7.36 | 99.3 | 5.53 | | Rutin | Y = 0.9379X − 0.0038 | 20.8–1040 | 0.9996 | 0.5 | 1.5 | 7.86 | 98.1 | 5.83 | | Epicatechin gallate | Y = 0.3410X − 0.0124 | 20.2–5050 | 0.9994 | 1 | 3 | 6.93 | 97.7 | 6.63 | | Luteolin-7-glucoside | Y = 1.6412X + 0.0779 | 20.2–1010 | 0.9998 | 0.1 | 0.3 | 2.64 | 105.0 | 4.28 | | Luteolin | Y = 21.2695X + 17.0019 | 20.2–1010 | 0.9990 | 0.1 | 0.3 | 2.35 | 102.5 | 3.71 | | Emodin | Y = 5.3529X + 0.0097 | 0.4–20 | 0.9997 | 0.003 | 0.01 | 0.00 | 104.3 | 2.24 | | Ursolic acid | Y = 0.1071X + 1.1690 | 7.92–198 | 0.9944 | 0.3 | 1.0 | 9.10 | 93.5 | 1.43 |
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