Lipemia Interferences in Biochemical Tests, Investigating the Efficacy of Different Removal Methods in comparison with Ultracentrifugation as the Gold Standard
Table 4
Comparison of the results of native serum, serum blank, and diluted serum with ultracentrifuged serum.
Parameter
Severe lipemia (TG > 1000 mg/dl)
Allowable bias
Native serum (%)
Serum blank (%)
Serum dilution (%)
ALT
0
11.48
Albumin
+13
0
+43
1.43
ALP
−3
6.72
Amylase
+14
+4
+15
7.4
AST
+14
−27
+36
6.54
Bilirubin (total)
0
8.95
Calcium
+40
+30
+57
0.82
Chloride
+82
+22
+90
0.5
CPK
−22
−5
−61
11.5
Creatinine
−20
−80
0
3.96
GGT
−9
11.6
Glucose
+44
+2
+63
2.34
Iron
−9
+660
+21
8.8
LDH
+9
+8
+7
4.3
Lipase
+7
11.31
Magnesium
+180
+108
+325
1.8
Phosphorus
+109
+187
+210
3.38
Total protein
+59
−3
+70
1.36
TIBC
+34
+33
+78
1.3
Urea
+11
+17
−17
5.57
Uric acid
+4
4.87
Difference with ultracentrifuged serum, calculated as bias.