Research Article
Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification
Table 1
Basic parameters of the LC-MS/MS system in this study.
| Parameter | Mode and setting value |
| Mobile phase A | Ammonium formate in water | Mobile phase B | Methanol | Flow rate | 0.15 mL/min | Injection volume | 10 µL | Column temperature | 30°C | Ionization | Electrospray ionization in positive mode (ESI+) | Capillary voltage | 2500 V | Desolvation gas temperature | 500°C | Desolvation gas flow | 800 L/h | Cone gas flow | 150 L/h | Collision gas flow | 0.15 mL/min | Nebulizer | 7 bar | Acquisition | Multiple reaction monitoring (MRM) |
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