Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification

Table 5

Calibration curves of B-group vitamins.

Compound	Calibration curve equation (A: ratio of native/internal standard signal, C: concentration ng/mL)	Coefficient of determination	Working range (ng/mL)	Bias (%)
THI	A = 1.196 × C + 0.752	0.9993	3–130	−5.6–5.7
RIB	A = 0.937 × C + 4.670	0.9990	10–200	−6.3–7.2
NIC	A = 1.825 × C + 6.585	0.9995	30–1200	−3.9–5.4
NIA	A = 1.178 × C + 0.959	0.9984	3–120	−3.9–5.5
PYN	A = 1.634 × C + 0.520	0.9992	3–140	−4.2–5.0
PYL	A = 3.200 × C + 0.385	0.9992	0.3–10	−3.6–4.0
PYM	A = 12.122 × C + 0.752	0.9994	0.3–10	−2.6–4.9