Development and Validation of Ecofriendly HPLC-MS Method for Quantitative Assay of Amoxicillin, Dicloxacillin, and Their Official Impurity in Pure and Dosage Forms

Table 5

Parameters of system suitability of the developed HPLC method for the determination of AMOX and DIC with their common impurity, 6-APA.

Parameters	AMOX	6-APA	DIC	Reference value [43]
Resolution (Rs)	1.427   1.544			>1.5
Selectivity (α)	1.576   1.380			>1
Capacity factor (K′)	0.610	1.538	2.503	1–10
Symmetry factor	0.916	0.889	1	T = 1 for a typical symmetric peak
Number of theoretical plates (N)	115.204	210.333	785.345	Increases with efficiency of the separation
HETP (cm/plate)	0.086	0.047	0.012	The smaller the value, the higher the column efficiency