A Direct and Sensitive Method for Determination of 5-Fluorouracil in Colorectal Cancer Cells: Evaluating the Effect of Stromal Cell on Drug Resistance of Cancer Cells

Figure 4

The concentration of 5-FU was measured in medium and in cells. (a) The concentration of 5-FU in the medium of 3T3 cells treated with 5-FU. (b) The concentrations of 5-FU in MC38 medium after a 24-hour treating with NIH3T3 CM. (c) The concentrations of 5-FU in NIH3T3 were determined after treatment with 5-FU. (d) The concentrations of 5-FU in MC38was determined 24 h after the treatment with NIH3T3 CM. (e) NIH3T3 increased the resistance of MC38 to 5-FU.

(a)

(b)

(c)

(d)

(e)