A Direct and Sensitive Method for Determination of 5-Fluorouracil in Colorectal Cancer Cells: Evaluating the Effect of Stromal Cell on Drug Resistance of Cancer Cells
Table 3
Interday and intraday precision and accuracy of 5-FU (n = 5).
Analyte
Nominal concentration (ng/mL)
Interday measured concentration (ng/mL) ±SD
RSD (%)
RE (%)
Intraday measured concentration (ng/mL) ±SD
RSD (%)
RE (%)
5-FU
20
18.41 ± 0.64
3.48
3.60
18.96 ± 1.04
5.50
3.74
50
52.90 ± 2.10
3.96
1.12
53.34 ± 1.68
3.15
1.34
500
524.94 ± 5.39
1.03
−0.77
518.36 ± 11.61
2.24
−0.79
4000
3791.21 ± 45.09
1.19
−0.96
4059.59 ± 102.83
2.47
−0.94
RE: relative error, which is calculated as (measured concentration − nominal concentration)/nominal concentration.
