A Fast and Validated HPLC Method for Simultaneous Determination of Dopamine, Dobutamine, Phentolamine, Furosemide, and Aminophylline in Infusion Samples and Injection Formulations

Table 1

Analytical parameters for DPM, DBM, PTM, FSM, and APL.

Analytical parameter	DPM	DBM	PTM	FSM	APL
λmax wavelength (nm)	280	271	279	279	276
Retention time(min)	3.93	5.84	7.06	8.76	9.67
Theoretical plate (mean ± SD)	5102 ± 86	34975 ± 1595	39586 ± 1724	27469 ± 1061	23985 ± 1362
Linear range (mg/L)	12.0 – 240.0	12.0 – 240.0	20.0 – 200.0	6.0 – 240.0	10.0 – 200.0
Linear equation	Y = 0.9892X − 2.0961	Y = 0.9672X + 0.9111	Y = 1.6493X + 1.4782	Y = 4.5218X + 32.275	Y = 2.9828X − 1.3333
Coefficient of correlation (r)	0.9999	0.9993	0.9995	0.9990	0.9998
Detection limit (mg/L)	0.48	0.12	0.20	0.24	0.18
Quantification limit (mg/L)	1.80	0.42	0.70	0.90	0.63