Single Standard Substance for the Simultaneous Determination of Eleven Components in the Extract of Paeoniae Radix Alba (Root of Paeonia lactiflora Pall.)

Table 8

The relative retention times (RRTs) of the components in different columns and instruments.

Relative correction factor	Column-Luna^a	Column-YMC^b	Column-BEH^c	Instrument-A^d	Instrument-U^e
RRTgallic acid/paeoniflorin	0.14	0.14	0.14	0.15	0.16
RRToxypaeoniflorin/paeoniflorin	0.65	0.62	0.34	0.62	0.69
RRTcatechin/paeoniflorin	0.69	0.67	0.37	0.67	0.73
RRTalbiflorin/paeoniflorin	0.92	0.92	0.78	0.92	0.93
RRTethyl gallate/paeoniflorin	1.04	1.02	0.93	1.04	1.05
RRTgalloylpaeoniflorin/paeoniflorin	1.21	1.23	1.44	1.19	1.21
RRTpentagalloylglucose/paeoniflorin	1.26	1.28	1.55	1.25	1.25
RRTbenzoic acid/paeoniflorin	1.27	1.24	1.26	1.27	1.28
RRTbenzoylpaeoniflorin/paeoniflorin	1.37	1.42	1.98	1.37	1.34
RRTpaeonol/paeoniflorin	1.48	1.51	1.99	1.50	1.46

^aThe Phenomenex Luna C18 column was used on the Waters HPLC system. ^bThe YMC-Pack Pro C18 column was used on the Waters HPLC system. ^cThe Waters BEH C18 column was used on the Waters HPLC system. ^dThe instrument was the Agilent 1260 system (Agilent Technologies Inc., Waldbornn, Germany) and the column was the Phenomenex Luna C18. ^eThe instrument was the Dionex UltiMate 3000 system (Thermo Scientific Inc., Germering, Germany) and the column was the Phenomenex Luna C18.