Research Article

LC-MS/MS Method for Determination of Hydroxychloroquine and Metabolites: Application in a Pharmacokinetic Study

Table 2

Extraction recovery and matrix effect data of the analytes in rat blood (n = 6).

AnalytesNominal concentration (ng/mL)Extraction recoveryMatrix effect
Mean ± SDRSD (%)Mean ± SDRSD (%)

BDCQ2.590.27 ± 12.9714.3766.20 ± 3.845.81
200086.64 ± 5.456.2983.14 ± 2.583.10

DCQ2.589.59 ± 10.9812.2675.87 ± 4.876.42
200087.14 ± 4.064.6685.15 ± 1.762.06

DHCQ2.586.42 ± 12.2414.1677.37 ± 3.264.21
200092.00 ± 3.673.9987.13 ± 1.832.10

HCQ592.14 ± 9.8610.7073.56 ± 2.793.79
400093.77 ± 4.284.5687.98 ± 1.441.64