Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells
Figure 11
Proportions of the fibroblasts with enlarged (hypertetraploidy) nuclei and with conventional small nuclei in the early presenescence stage, estimated in one of the samples by the labelling frequency for γ-H2AX ( cells), NANOG/p16 positive ( cells), and NANOG/p21-positive nuclear granules ( cells). All nuclear labels were mostly present in this phase in the perinucleolar region. Evaluation reveals a many-fold prevailing signalling for DDR, self-renewal, and senescence regulators occurring simultaneously in the enlarged hypertetraploidy cells, which left normal cell cycle.
