Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells
Figure 9
Characteristic cytological nuclear IF features of the tetraploid cells in the presenescence phase showing synergism of several labels located near the nucleoli. (a) Initial DDR in the perinucleolar chromatin indicated by the γ-H2AX-positive label (arrowheads), NL-nucleolus. (b) The accumulation of p16 and NANOG-positive, partly colocalising granules in the perinucleolar region (arrowheads). (c) The accumulation of p21 and NANOG—partial colocalisation around the central nucleolus (arrowheads). (d) The emergence of initial gentle SAHFs revealed by DNA-specific metachromatic staining with Toluidine-blue in the perinucleolar chromatin (arrowheads). (e) PML bodies in the vicinity of the central lobulated nucleolus, where they partly colocalise with γ-H2AX-foci. (f) The tetraploid cell from the mid-presenescence with the network of γ-H2AX and some amount of NANOG, partially colocalised. Images were obtained using BRG three-colour optical filters system. Scale bars =10 μm.
