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Journal of Aging Research
Volume 2011, Article ID 103253, 14 pages
http://dx.doi.org/10.4061/2011/103253
Research Article

Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells

1Latvian Biomedical Research and Study Centre, Ratsupites 1, 1067 Riga, Latvia
2Latvian Institute of Organic Synthesis, Aizkraukles 21, 1006 Riga, Latvia

Received 15 December 2010; Revised 22 February 2011; Accepted 25 February 2011

Academic Editor: Noam Shomron

Copyright © 2011 Anda Huna et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental figures showing on (1–6) six microscopic fields of the DNA content measurements by integral fluorescence in the DAPI channel using Image Pro Plus software including cells with enlarged nuclei (asterix) and stained for: (a,b) NANOG, p16 and DNA by DAPI. The measurements show that the enlarged nuclei simultaneously expressing the initial self–renewal and senescence landmarks are para–tetraploid, hyper–tetraploid, and octaploid (cycling). On Figure 7 confocal pictures (separation) are presented as obtained from the early pre–senescence sample stained for NANOG (green), p21 (red) and DNA by DAPI (blue), scanned in the colour channels in sequence. (a) DAPI channel (DNA); (b) NANOG; (c) p16; (d) merged. A central cell with enlarged nucleus has peri–nucleolar accumulation of the NANOG and p16 positive granules, partly co–localised. NL—the nucleolus.

  1. Supplementary Material