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Journal of Aging Research
Volume 2011, Article ID 393416, 11 pages
Research Article

Interactions of Aging, Overload, and Creatine Supplementation in Rat Plantaris Muscle

1Department of Anatomy, College of Osteopathic Medicine, University of New England, Biddeford, ME 04005, USA
2Department of Physiological Sciences, Stellenbosch University, Stellenbosch 7602, South Africa
3Ohio Musculoskeletal and Neurological Institute (OMNI), Department of Biomedical Science, Ohio University College of Osteopathic Medicine, Athens, OH 45701, USA

Received 18 March 2011; Accepted 19 May 2011

Academic Editor: Paula Bickford

Copyright © 2011 Mark D. Schuenke et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Attenuation of age-related sarcopenia by creatine supplementation has been equivocal. In this study, plantaris muscles of young (Y; 5m) and aging (A; 24m) Fisher 344 rats underwent four weeks of either control (C), creatine supplementation (Cr), surgical overload (O), or overload plus creatine (OCr). Creatine alone had no effect on muscle fiber cross-sectional area (CSA) or heat shock protein (HSP70) and increased myonuclear domain (MND) only in young rats. Overload increased CSA and HSP70 content in I and IIA fibers, regardless of age, and MND in IIA fibers of YO rats. CSA and MND increased in all fast fibers of YOCr, and CSA increased in I and IIA fibers of AOCr. OCR did not alter HSP70, regardless of age. MND did not change in aging rats, regardless of treatment. These data indicate creatine alone had no significant effect. Creatine with overload produced no additional hypertrophy relative to overload alone and attenuated overload-induced HSP70 expression.