Identification of Potential Calorie Restriction-Mimicking Yeast Mutants with Increased Mitochondrial Respiratory Chain and Nitric Oxide Levels
Figure 3
Replicative lifespan (RLS) analysis of potential CR genetic mimics. (a) Potential CR mimicking high-NO mutants show extended RLS and do not significantly further increase the RLS of cells grown in CR. (b) CR decreases soy1Δ-induced RLS (soy1Δ versus soy1Δ, CR; ); however, soy1Δ does not significantly change CR-induced RLS (CR versus soy1Δ, CR; ). (c) CR further increases gup1Δ-induced RLS (gup1Δ versus gup1Δ, CR; ). gup1Δ also further increases CR-induced RLS (CR versus gup1Δ, CR; ). (d) Although hhf1Δ further increases CR-induced RLS (CR versus hhf1Δ, CR; ), CR does not further increase hhf1Δ-induced RLS (hhf1Δ versus hhf1Δ, CR; ). One representative set of three independent experiments is shown. WT: BY4742 wild-type control; CR: 0.5% glucose; Δ: gene deletion. Pairwise statistical analysis of RLS is shown in Supplemental Table 3.
