Low-Dose, Ionizing Radiation and Age-Related Changes in Skeletal Microarchitecture
Figure 3
Bone-cell differentiation potential as a function of age and radiation exposure. Hindlimb bone marrow was flushed and cultured under osteoblastogenic or osteoclastogenic conditions to assess differentiation potential of the marrow. Between 1 and 4 months of aging (white columns), osteoblastogenesis (a), measured as the area of calcified matrix per well, and osteoclastogenesis (b), measured as number of TRAP-positive, multinuclear (≥3/cell) cells per area, remained constant in sham-control mice. Irradiation, at any dose (gray or black columns), did not affect the differentiation potential of bone cells in the marrow at 1 (not shown) or 4 months post-IR (a, b). Images of alizarin red-stained mineralized nodules (c) and TRAP-stained osteoclasts (d).
