Letrozole Potentiates Mitochondrial and Dendritic Spine Impairments Induced by β Amyloid

Figure 3

Letrozole and 𝐴𝛽_1--42 cause significant changes in mitochondrial morphology. (a) Representative 3D reconstructions of MitoTracker Deep Red FM-positive mitochondria from hippocampal dendritic segments treated with 𝐴𝛽_1--42 (1 μM) for 24 hours in the presence and absence of 3-day letrozole (1 μM) treatment. Scale bar, 5 μm. (b) Quantifications of mitochondrial volumes. Significant differences in mitochondrial volume, compared to control cultures (4.03±0.56 μm³), were observed for letrozole- (1.72±0.49 μm³, 𝑃=0.015) treated cultures, as indicated by (*). Although there is no significant difference in mitochondrial volume between control and 𝐴𝛽_1--42 (𝑃=0.25), it is noteworthy that further reduction in mitochondrial volume relative to 𝐴𝛽_1--42 (2.88±0.32 µm³) was observed in letrozole + 𝐴𝛽_1--42 treated cultures (0.86±0.33 µm³, 𝑃=3.37×10⁻⁴) and is signified by (#).

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