Figure 3: Letrozole and cause significant changes in mitochondrial morphology. (a) Representative 3D reconstructions of MitoTracker Deep Red FM-positive mitochondria from hippocampal dendritic segments treated with (1 μM) for 24 hours in the presence and absence of 3-day letrozole (1 μM) treatment. Scale bar, 5 μm. (b) Quantifications of mitochondrial volumes. Significant differences in mitochondrial volume, compared to control cultures ( μm3), were observed for letrozole- ( μm3, ) treated cultures, as indicated by (*). Although there is no significant difference in mitochondrial volume between control and (), it is noteworthy that further reduction in mitochondrial volume relative to ( µm3) was observed in letrozole + treated cultures ( µm3, ) and is signified by (#).