Knockout of Vasohibin-1 Gene in Mice Results in Healthy Longevity with Reduced Expression of Insulin Receptor, Insulin Receptor Substrate 1, and Insulin Receptor Substrate 2 in Their White Adipose Tissue

Figure 3

Downregulation of insr, irs-1, and irs-2 in WAT of young vash1^−/− mice. (a) Total RNA was isolated from the WAT of WT and vash1^−/− young male mice, and the expression of insr, irs-1, irs-2, and Foxo1 was compared between WT and vash1^−/− mice. (b) Total RNA was isolated from skeletal muscle or liver of WT and vash1^−/− young male mice; and the expression of insr, irs-1, and irs-2 was compared between WT and vash1^−/− mouse tissues. (c) Total RNA was isolated from WAT, liver, and skeletal muscle of young WT male mice, and the expression of vash1 was compared. In (a)–(c), the means ± SDs are shown, the statistical significance of differences was calculated by use of the unpaired Student’s -test, and a value of was the criterion for significance. .

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