Age-Dependent Loss of Mitochondrial Function in Epithelial Tissue Can Be Reversed by Coenzyme Q₁₀

Figure 1

Microscopic image of an epidermis in an XF24 islet capture microplate. The epidermis was applied as flat as possible with the basal side on the net and then placed in the multiwell plate. The measurement of oxygen consumption takes place within the ring marked with the arrow (a). It was especially critical to ensure after detachment of the epidermis from the dermis that the basal side shows to the net. In addition, within the ring in the center of the net, there should be no holes (b) or air bubbles (c). Normalization of the obtained data takes place over the surface within the inner ring of the net (d). Schematic setup of the XF24 measuring system with an islet capture microplate (e).