Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy
PINK1 silencing inhibited mitophagy in SMC. P20 SMCs were transfected with 5 nM PINK1 siRNA or scrambled siRNA (control), and after 48 hours, cells were exposed to IGF-1, bafilomycin (Baf), or combination of IGF-1 + Baf. Mitophagy was determined by quantifying of number of mitophagic vacuoles/cell (a) or by assessing of mitochondrial LC3 II/I ratio (b). Black bars, control siRNA-transfected cells; grey bars, PINK1 siRNA-transfected cells. PINK1 siRNA vs. control siRNA; # for SMC with IGF-1 vs. no IGF-1.
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