Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris)

Figure 3

Con A staining without (−Man) and in presence of 10 mM mannose (+Man). Both blots show N. sylvestris seed protein after size exclusion chromatography with (R) and without (NR) 𝛽-ME. A and B indicate position of vicilin bands used for the TFA hydrolysis and subsequent HPAEC-PAD carbohydrate analysis. 4–15% Tris-HCl gel transferred to PVDF.