Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (<i>Nicotiana sylvestris</i>)

<table>Cross-reactivity of anti-<i>G. max</i> IgG. Lane M, mass marker; lane 1, total soluble protein from <i>N. sylvestris</i>; lane 2, 11S-enriched fraction from <i>N. sylvestris</i>; lane 3, 7S (vicilin)-enriched fraction from <i>N. sylvestris</i>; lane 4, total soluble protein from <i>C. maxima</i>; lane 5, total soluble protein from <i>G. max</i>; lane 6, bovine serum albumin; lane 7, bovine fetuin; lane 8, mixed animal and plant protein control consisting of human transferrin (<svg height="5.7375002" id="M58" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.1 5.7375002" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,5.675)"><path d="M548 311q-21 -57 -59 -94t-89 -37q-53 0 -128 52q-55 38 -84 38q-39 0 -70.5 -23t-45.5 -60l-32 12q21 57 59 94t89 37q53 0 128 -52q55 -38 84 -38q39 0 70.5 23t45.5 60z" id="x223C"></path></g>
</svg>75 kDa), bovine serum albumin (<svg height="5.7375002" id="M59" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.1 5.7375002" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,5.675)"><path d="M548 311q-21 -57 -59 -94t-89 -37q-53 0 -128 52q-55 38 -84 38q-39 0 -70.5 -23t-45.5 -60l-32 12q21 57 59 94t89 37q53 0 128 -52q55 -38 84 -38q39 0 70.5 23t45.5 60z" id="x223C"></path></g>
</svg>62 kDa), asialofetuin (<svg height="5.7375002" id="M60" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.1 5.7375002" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,5.675)"><path d="M548 311q-21 -57 -59 -94t-89 -37q-53 0 -128 52q-55 38 -84 38q-39 0 -70.5 -23t-45.5 -60l-32 12q21 57 59 94t89 37q53 0 128 -52q55 -38 84 -38q39 0 70.5 23t45.5 60z" id="x223C"></path></g>
</svg>50 kDa), peanut lectin (<svg height="5.7375002" id="M61" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.1 5.7375002" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,5.675)"><path d="M548 311q-21 -57 -59 -94t-89 -37q-53 0 -128 52q-55 38 -84 38q-39 0 -70.5 -23t-45.5 -60l-32 12q21 57 59 94t89 37q53 0 128 -52q55 -38 84 -38q39 0 70.5 23t45.5 60z" id="x223C"></path></g>
</svg>25 kDa), and ribonuclease B (<svg height="5.7375002" id="M62" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 10.1 5.7375002" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,5.675)"><path d="M548 311q-21 -57 -59 -94t-89 -37q-53 0 -128 52q-55 38 -84 38q-39 0 -70.5 -23t-45.5 -60l-32 12q21 57 59 94t89 37q53 0 128 -52q55 -38 84 -38q39 0 70.5 23t45.5 60z" id="x223C"></path></g>
</svg>20 kDa). Arrow indicates position of homologous allergens from <i>G. max</i> (23 kDa peptide from Bd 28 K) and <i>C. maxima</i> (MP27/MP32). 4–12% Bis-Tris gel transferred to PVDF.</table>

Journal of Botany

fig6

Figure 6

Figure 6: Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (<i>Nicotiana sylvestris</i>) 

Journal of Botany

Partial Characterization of a Vicilin-Like Glycoprotein from Seeds of Flowering Tobacco (Nicotiana sylvestris)

Figure 6