Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR

Table 2

Validation of the relatively equivalent of (RT-) PCR amplification efficiencies between the target and the candidate housekeeping gene.

	ACTα	ARPT	CYP	EF1  𝛼	EIF1  𝛼	GAPD	rbcL	TUBα	TUBβ	UBQ
IPPI	−0.079^∗	−0.185	−0.448	−0.083^∗	−0.266	0.702	−0.418	−0.205	−0.562	−0.480
FPPS1	0.017^∗	−0.088	−0.351	0.013^∗	−0.169	0.772	−0.322	−0.109	−0.465	−0.384

Data are based on linear regression analyses using ΔCt values (Ct target gene - Ct housekeeping gene) from the samples for standard curve versus log concentrations of standard curve; *: slope of semilog regression line < 0.1.