Improved and Reproducible Flow Cytometry Methodology for Nuclei Isolation from Single Root Meristem

Figure 2

Representative flow histograms of DAPI-stained nuclei from Allium cepa root meristem after 2- (a), 4- (b), 6- (c), and 8- (d) pulse homogenization tests, showing in 𝐺₀/𝐺₁ peaks: 4120 nuclei (CV 9.16%), 3855 nuclei (CV 7.36%), 4723 nuclei (CV 3.2%) and 2685 nuclei (CV 6.47%), respectively. Note that the most adequate histogram corresponds to 6-pulse homogenization (c).

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(b)

(c)

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