Analysis of Genetic Diversity in Astragalus rhizanthus Benth. ssp. rhizanthus var. rhizanthus (Fabaceae) Using Molecular Markers from India

Table 2

ISSR, RAPD, and DAMD primers used for PCR profiling of DNA of A. rhizanthus ssp. rhizanthus var. rhizanthus and the extent of polymorphism detected with these primers.

Primer	Sequence (5^′-3^′)	No. of loci amplified	Polymorphic bands	% Polymorphism
ISSR
808	(AG)8C	18	17	94.44
809	(AG)8G	20	19	95
810	(GA)8T	19	18	94.73
811	(GA)8C	18	17	94.44
812	(GA)8A	18	17	94.44
816	(CA)8T	21	20	95.23
817	(CA)8A	21	20	95.23
818	(CA)8G	13	12	92.30
823	(TC)8C	14	13	92.85
824	(TC)8G	11	7	63.63
825	(AC)8T	15	14	93.33
830	(TG)8G	23	22	95.65
840	(GA)8CT	17	15	88.23
848	(CA)8AG	10	10	100
855	(AC)8CT	22	21	95.45
RAPD
OP-B06	TGCTCTGCCC	22	20	90.91
OP-B07	GGTGACGCAG	21	19	90.47
OP-B08	GTCCACACAG	18	15	83.33
OP-B09	TGGGGGACTC	18	17	94.44
OP-C05	GATGACCGCC	17	16	94.12
OP-C15	GACGGATCAG	17	17	100
OP-N06	GAGACGCACA	23	23	100
OP-N08	ACCTCAGCTC	25	20	80
OP-N10	ACAACTGGGG	14	12	85.71
OP-N17	CATTGGGGAG	21	20	95.23
OP-N18	GGTGAGGTCA	24	22	91.66
OP-N19	GTCCGTACTG	23	21	91.30
OP-N20	GGTGCTCCGT	20	19	95
OP-U01	ACGGACGTCA	25	24	96
OP-U07	CCTGCTCATC	26	25	96.15
OP-U08	GGCGAAGGTT	24	23	95.83
OP-U10	ACCTCGGCAC	21	18	85.71
OP-U12	TCACCAGCCA	22	21	95.45
DAMD
M13	GAGGGTGGCGGTTCCT	25	23	92
33.6	GGAGGTTTTTCA	25	21	88
HVR	CCTCCTCCCTCCT	22	20	90.90
HVA	AGGATGGAAAGGAGGC	32	29	90.62
HVY	GCCTTTCCCGAG	26	25	96.15
HVV	GGTGTAGAGAGGGGT	26	24	92.30