Leaves and Seeds as Materials for Flow Cytometric Estimation of the Genome Size of 11 Rosaceae Woody Species Containing DNA-Staining Inhibitors

Figure 2

Histograms of PI fluorescence intensity (a, d) and dot plots on side scatter (SSC) versus PI fluorescence (b, e) and forward scatter (FSC) versus SSC (c, f) in nuclei of leaves of C. divaricatus and Z. mays (internal standard) isolated using Galbraith’s buffer (a–c) and Galbraith’s buffer supplemented with 2% (w/v) PVP-10 and 15 mM 𝛽-mercaptoethanol. C. divaricatus does not possess the 4C nuclei that coincide with the 2C peak of Z. mays. Arrows indicate additional populations of particles (see text for explanation).

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