Cell Wall Growth and Modulation Dynamics in a Model Unicellular Green Alga—Penium margaritaceum: Live Cell Labeling with Monoclonal Antibodies

Figure 2

Live cell labeling (JIM5) of single cells incubated under different experimental conditions. Except for cells grown normal light conditions (3,000 lux), the images of the other cells were from 28 h cultures. Low light conditions (100 lux) produced virtually no wall expansion while high light (5,400 lux) produced cells with asymmetric labeling patterns. Incubation of cells in medium adjusted to pH 5 or pH 9 resulted in little alteration to cell expansion. Cells incubated in 4% mannitol, glucose, or sucrose resulted in significant cell expansion.