Research Article

Role of Ascorbate in the Regulation of the Arabidopsis thaliana Root Growth by Phosphate Availability

Figure 4

Effect of phosphate availability and ascorbate concentration on the cytokinesis frequency in the root tips of Arabidopsis thaliana. Mitotic activity in the root tips of wild-type (Col 0) and vtc1 seedlings was analyzed after 8 and 12 days of culture on the media containing low (1 μM) P or high (1 mM) P concentration. Wild-type seedlings were cultured on media supplemented with varying concentrations of ASC or on ascorbate—free media (control). Seedlings of vtc1 mutant were cultured on ascorbate—free media. Each determination is based on at least three squash preparations. Mean value and ±SD are indicated. Data represent mean and standard deviation. Asterisk indicates significant differences from the control at 𝑃 < 0 . 0 5 .
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