Role of Ascorbate in the Regulation of the Arabidopsis thaliana Root Growth by Phosphate Availability

Table 2

Effect of phosphate availability and exogenous ASC on ascorbate redox state (([ASC]/[ASC+DHA])×100) in root apices of Arabidopsis seedlings.

1 μM PO43−
control	300 μM ASC	500 μM ASC	vtc1
83,3±5,0	78,9±7,9	87,5±7,8	61,1±8,9^∗
1 mM PO43−
control	300 μM ASC	500 μM ASC	vtc1
60,9±7,2	77,6±7,2^∗	83,3±5,1^∗	58,2±4,7

Ascorbate redox state (([ASC]/[ASC+DHA])×100) in root tips of Arabidopsis seedlings was determined after 12 days of culture. Arabidopsis seedlings were grown on media containing 1 μM PO₄₃₋ or 1 mM PO₄₃₋ or on the same media supplemented with 100 or 500 μM ASC. Seedlings of vtc1 mutant were grown on medium containing either 1 μM PO₄₃₋ or 1 mM PO₄₃₋. *Significant differences from control with 𝑃<0.05.