Agrobacterium tumefaciens T-DNA Integration and Gene Targeting in Arabidopsis thaliana Non-Homologous End-Joining Mutants
Figure 4
Comet assay. (a) The fraction of DNA in comet tails (%DNA in tail) was used as a measure of DNA damage in wild-type plants (WT), Atku70 and Atku80 mutants: no treatment (black bars); 2 h bleomycin treatment (white bars); 24 h bleomycin treatment (dark grey bars); 24 h bleomycin treatment followed by 24 h recovery (light grey bars). For each treatment, around 100 nuclei were analyzed. (b) According to the value of % DNA in tail, nuclei from the plants after the treatments indicated in (a) were divided into three groups: <5% damage (light grey bars); 5–10% damage (dark grey bars); >10% damage (white bars).
